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SARS-CoV-2

SARS-CoV-2 or COVID 19

The World Health Organisation (WHO) was informed about a cluster of pneumonia cases of unknown aetiology in Wuhan City, Hubei Province, China on 31 December 2019. On the 7th of January 2020, a Novel Coronavirus (2019-nCoV) was identified as the causative virus by the Chinese authorities. The Novel Coronavirus is formally named SARS-CoV-2. The disease caused by the virus is now referred to as COVID-19.​ Since then, the virus has spread to more than 100 countries.

SARS-CoV-2 is an enveloped, positive sense, single-stranded ribonucleic acid (RNA) virus with a 30-kb genome. The virus has an RNA proofreading mechanism keeping the mutation rate relatively low. The genome encodes for non-structural proteins and four structural proteins. SARS-CoV-2 is the seventh coronavirus identified that is known to infect humans.

The clinical presentation of SARS-CoV-2 infection can range from asymptomatic infection to severe disease. Mortality rates differ per country. Early laboratory diagnosis of a SARS-CoV-2 infection can aid clinical management and outbreak control.

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GLOBAL CASES OF COVID-19

COVID 19 PCR BASED DIAGNOSTIC TESTING

The gold standard for detecting SARS-CoV-2 is a polymerase chain reaction (PCR). This tests for the genetic material of the virus, and is performed most commonly on a swab taken from the nose and throat, or from sputum (mucus from the lungs) in unwell patients.

Quantitative RT-PCR monitors the amplification of the virus during the course of the PCR (i.e., in real time), not at its end. The method is used to estimate the viral load in a patient. Should the detection exceed a certain threshold, the patient will be positive for SARS-CoV-2.

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VIRAL RNA EXTRACTION KITS FOR COVID 19

  • Several Viral RNA extraction kits for manual and automated workflows for extractions from swabs.

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  • The purified product is suitable for all downstream applications such as RT-PCR.

  • Download sales sheets below for an overview of each workflow and kit contents.

  • Magnetic bead based

  • Suitable for 8 - 5000 samples

  • For bench top workflows using single or multi-channel pipettes, or high through-put automated systems

  • Locally manufactured, short lead-times

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Bottom up Mag-tech

Third-party platform

Seegene Nimbus

184 samples per kit

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Top down Mag-tech

Third-party platform

 KingFisher Flex

1000 or 5000 samples per kit

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Manual Mag-tech

Multichannel pipette only

1000 samples per kit

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  • Silica spin column based

  • Single column format

    • For 1 - 24 samples

    • Can process up to 48 samples at once

    • Influenced by rotor size of your centrifuge

  • 96 well plate format

    • For 96 - 3000 samples per day

    • Requires vacuum manifold or centrifuge bucket rotor

    • Fast and easy

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Single spin column format

100 samples per kit

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96 well format (mini)

350 ul volume, 384 samples per kit

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96 well format (maxi)

1000 ul, 384 samples per kit